Journal: CNS Neuroscience & Therapeutics

Article Title: Astaxanthin Inhibits Ferroptosis of Hippocampal Neurons in Kainic Acid‐Induced Epileptic Mice by Activating the Nrf2/ GPX4 Signaling Pathway

doi: 10.1111/cns.70238

Figure Lengend Snippet: AST administration ameliorates neuronal loss and cognitive impairment in KA‐induced epileptic mice. (A) Representative Nissl staining images of hippocampal tissues from the three groups of mice (bar = 10 μm) with statistical data ( n = 3). (B) Representative track plots on the fifth day of the Morris water maze test for the three groups of mice. (C) Swimming speed of the three groups of mice. (D) Platform crossings on the fifth day of the Morris water maze test for the three groups of mice. (E) Escape latency during training for the three groups of mice ( n = 5). * indicates comparison between sham and KA groups, # indicates comparison between KA and KA + AST groups. (F) Schematic of the novel object recognition test. (G) Recognition index in the novel object recognition test for the three groups of mice ( n = 5). Data are presented as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Brain sections (5 μm) were incubated with Nissl staining solution (C0117, Beyotime, Shanghai, China) for 15 min. Standard processing was performed, and images were captured using a Nikon optical microscope (Nikon, Tokyo, Japan).

Techniques: Staining, Comparison

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Paeoniflorin Attenuates Dexamethasone-Induced Apoptosis of Osteoblast Cells and Promotes Bone Formation via Regulating AKT/mTOR/Autophagy Signaling Pathway

doi: 10.1155/2021/6623464

Figure Lengend Snippet: Effect of paeoniflorin on DEX-induced apoptosis in MC3T3-E1 cells. (a, b) The extent of apoptosis of MC3T3-E1 cells was detected by flow cytometry after Annexin V-FITC/PI double staining. The apoptotic rate was measured by flow cytometric analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX. (c) MC3T3-E1 cells were stained by Hoechst to show the extent of apoptosis after interventions of DEX and paeoniflorin. (d) The expressions of osteogenic protein-Runx2 (runt-related transcription factor2) and apoptotic proteins such as Bcl-2 (B cell leukemia 2) and Bax (BCL2-associated X protein) were measured by western blot. ∗ P < 0.05, ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX; ## P < 0.01 vs. DEX. (e) Protein levels of caspase-3 and cleaved caspase-3 were determined by western blot analysis, shown in quantitative analysis. Data are presented as the mean ± SD. ∗∗ P < 0.01 vs. control; # P < 0.05 vs. DEX.

Article Snippet: For Hoechst staining, 10 μ L Hoechst live cell staining solution (Beyotime Institute of Biotechnology) was added to the culture medium and mixed gently.

Techniques: Flow Cytometry, Double Staining, Control, Staining, Western Blot